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dmem f12 culture medium  (Beijing Solarbio Science)


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    Beijing Solarbio Science dmem f12 culture medium
    Dmem F12 Culture Medium, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dmem+f12+culture+medium/DMEM%2FF12+Culture+Medium/pmc12540828-335-5-14
    Average 93 stars, based on 21 article reviews
    dmem f12 culture medium - by Bioz Stars, 2026-09
    93/100 stars

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    Incubation:

    Article Title: Electroporation-based Easi-CRISPR yields biallelic insertions of EGFP-HiBiT cassette in immortalized chicken oviduct epithelial cells.
    Article Snippet: .. First, cOECs were incubated for 5 h in DMEM/F12 culture medium containing the retrovirus and 4 mg/mL of polybrene (Solarbio). .. Then, the culture medium was replaced with fresh medium containing 1.0 mg/mL of puromycin (SigmaAldrich).

    In Vitro:

    Article Title: NADase CD38 is a key determinant of ovarian aging
    Article Snippet: For ovarian LPS treatments in vivo, 8-month-old female mice were injected intraperitoneally with LPS (Sigma) at a concentration of 1 mg ml −1 based on a previous study , and then the ovaries were collected after 24 h for subsequent experiments. .. For LPS treatment in vitro, ovaries from 10-day-old mice were placed on culture plate inserts (Millipore Corporation) and cultured in 400 μl of DMEM/F12 culture medium (Solarbio) supplemented with 10% FBS (Biological Industries) and 1% penicillin–streptomycin 100× solution (HyClone) in an incubator at 37 °C with 5% CO 2 as previously reported . ..

    Article Title: NADase CD38 is a key determinant of ovarian aging.
    Article Snippet: For ovarian LPS treatments in vivo, 8-month-old female mice were injected intraperitoneally with LPS (Sigma) at a concentration of 1 mg ml−1 based on a previous study68, and then the ovaries were collected after 24 h for subsequent experiments. .. For LPS treatment in vitro, ovaries from 10-day-old mice were placed on culture plate inserts (Millipore Corporation) and cultured in 400 μl of DMEM/F12 culture medium (Solarbio) supplemented with 10% FBS (Biological Industries) and 1% penicillin–streptomycin 100× solution (HyClone) in an incubator at 37 °C with 5% CO2 as previously reported69. .. For LPS treatment in vitro, ovaries from 10-day-old mice were placed on culture plate inserts (Millipore Corporation) and cultured in 400 μl of DMEM/F12 culture medium (Solarbio) supplemented with 10% FBS (Biological Industries) and 1% penicillin–streptomycin 100× solution (HyClone) in an incubator at 37 °C with 5% CO2 as previously reported69.

    Cell Culture:

    Article Title: NADase CD38 is a key determinant of ovarian aging
    Article Snippet: For ovarian LPS treatments in vivo, 8-month-old female mice were injected intraperitoneally with LPS (Sigma) at a concentration of 1 mg ml −1 based on a previous study , and then the ovaries were collected after 24 h for subsequent experiments. .. For LPS treatment in vitro, ovaries from 10-day-old mice were placed on culture plate inserts (Millipore Corporation) and cultured in 400 μl of DMEM/F12 culture medium (Solarbio) supplemented with 10% FBS (Biological Industries) and 1% penicillin–streptomycin 100× solution (HyClone) in an incubator at 37 °C with 5% CO 2 as previously reported . ..

    Article Title: Moringa oleifera leaf extracts protect BMSC osteogenic induction following peroxidative damage by activating the PI3K/Akt/Foxo1 pathway.
    Article Snippet: .. To induce commitment toward an osteogenic lineage, P3 cells were cultured in osteogenic differentiation induction (OS) medium, which was comprised of DMEM/ F12 culture medium supplemented with fetal bovine serum (10%), 50 μg/ml vitamin C, 10 mmol/L βglycerophosphate, and 0.1 μmol/L dexamethasone (Solarbio; Beijing, China), as previously reported [29]. ..

    Article Title: NADase CD38 is a key determinant of ovarian aging.
    Article Snippet: For ovarian LPS treatments in vivo, 8-month-old female mice were injected intraperitoneally with LPS (Sigma) at a concentration of 1 mg ml−1 based on a previous study68, and then the ovaries were collected after 24 h for subsequent experiments. .. For LPS treatment in vitro, ovaries from 10-day-old mice were placed on culture plate inserts (Millipore Corporation) and cultured in 400 μl of DMEM/F12 culture medium (Solarbio) supplemented with 10% FBS (Biological Industries) and 1% penicillin–streptomycin 100× solution (HyClone) in an incubator at 37 °C with 5% CO2 as previously reported69. .. For LPS treatment in vitro, ovaries from 10-day-old mice were placed on culture plate inserts (Millipore Corporation) and cultured in 400 μl of DMEM/F12 culture medium (Solarbio) supplemented with 10% FBS (Biological Industries) and 1% penicillin–streptomycin 100× solution (HyClone) in an incubator at 37 °C with 5% CO2 as previously reported69.

    Centrifugation:

    Article Title: The PKM2/HIF-1α Axis is Involved in the Pathogenesis of Endometriosis via TGF-β1 under Endometrial Polyps.
    Article Snippet: Fresh EP samples collected from patients with EP with or without endometriosis under sterile conditions were minced finely and digested enzymatically with 5 mg of collagenase I (500 μg/mL; #17100017; GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA) and 1 mg deoxyribonuclease type I (100 μg/mL; #10325ES80; Yeasen, Shanghai, China) for 1 h at 37 °C. .. After centrifugation, the cells were suspended in DMEM/F12 culture medium containing 1 nM estradiol (#IE0210; Solarbio, Beijing, China), 0.2% insulin (#P3376; Beyotime), 1% L-glutamine (#ST1441-25g; Beyotime), 1% antibiotic solution (#SNA001; Sunncell, Wuhan, China), and 10% dextran-coated charcoal-treated fetal bovine serum (#SH30068.03; HyClone, Logan, UT, USA). .. The purity of the isolated ESCs was confirmed >95% (P2-P3) by Immunocytochemistry (ICC) staining using antibodies against vimentin (stromal cell marker) (#FNab09409; Finetest, Wuhan, China) and cytokeratin (epithelia cell marker) (#bs-1712R; Bioss).

    Suspension:

    Article Title: Fexofenadine protects against osteoarthritis by targeting Smad2 and STAT1 to enhance anabolism and binding cPLA2 to inhibit catabolism
    Article Snippet: .. The tissue was digested in DMEM/F12 culture medium supplemented with 0.25% collagenase II (C8150; Beijing Solarbio) at 37 °C with 5% CO 2 for 4–6 h. The resulting cell suspension was collected and seeded in six-well plates for further experiments. ..



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    Myogenic and adipogenic induction of TCCF2022 cells. ( a ) The relative expression of Myf5 in TCCF2022 after the addition of Forskolin, LY411575 , RepSox, and CHIR99021, respectively, on the basis of 5-Aza treatment was detected by qRT-PCR. ( b ) The relative expression of Myha in TCCF2022 cells pre-treated with a small-molecule compound after low-serum induced differentiation was detected by qRT-PCR. Statistical analysis was performed on relevant data using one-way ANOVA. Error bars indicate s.d.; ns p ≥ 0.05; * p < 0.05; *** p < 0.0005; **** p < 0.00005. ( c ) Forskolin-treated cells were treated with 15 μM 5-Azacytidin, 10μM Forskolin, 10 nM LY411575 , 5 μM RepSox, and 2 μM CHIR99021 for 2 days, and F12 medium containing 2% FBS for 2 days, and the results of bright field and immunofluorescence are shown in the experimental group. Scale bars: 80 µm. ( d ) Forskolin-treated cells were treated <t>with</t> <t>DMEM/F12</t> medium containing 8% HS for 3 days, and the results of bright field and Oil red O staining are shown in the experimental group. Scale bars: 80 µm.
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    Image Search Results


    Myogenic and adipogenic induction of TCCF2022 cells. ( a ) The relative expression of Myf5 in TCCF2022 after the addition of Forskolin, LY411575 , RepSox, and CHIR99021, respectively, on the basis of 5-Aza treatment was detected by qRT-PCR. ( b ) The relative expression of Myha in TCCF2022 cells pre-treated with a small-molecule compound after low-serum induced differentiation was detected by qRT-PCR. Statistical analysis was performed on relevant data using one-way ANOVA. Error bars indicate s.d.; ns p ≥ 0.05; * p < 0.05; *** p < 0.0005; **** p < 0.00005. ( c ) Forskolin-treated cells were treated with 15 μM 5-Azacytidin, 10μM Forskolin, 10 nM LY411575 , 5 μM RepSox, and 2 μM CHIR99021 for 2 days, and F12 medium containing 2% FBS for 2 days, and the results of bright field and immunofluorescence are shown in the experimental group. Scale bars: 80 µm. ( d ) Forskolin-treated cells were treated with DMEM/F12 medium containing 8% HS for 3 days, and the results of bright field and Oil red O staining are shown in the experimental group. Scale bars: 80 µm.

    Journal: Foods

    Article Title: Fin Cells as a Promising Seed Cell Source for Sustainable Fish Meat Cultivation

    doi: 10.3390/foods14122075

    Figure Lengend Snippet: Myogenic and adipogenic induction of TCCF2022 cells. ( a ) The relative expression of Myf5 in TCCF2022 after the addition of Forskolin, LY411575 , RepSox, and CHIR99021, respectively, on the basis of 5-Aza treatment was detected by qRT-PCR. ( b ) The relative expression of Myha in TCCF2022 cells pre-treated with a small-molecule compound after low-serum induced differentiation was detected by qRT-PCR. Statistical analysis was performed on relevant data using one-way ANOVA. Error bars indicate s.d.; ns p ≥ 0.05; * p < 0.05; *** p < 0.0005; **** p < 0.00005. ( c ) Forskolin-treated cells were treated with 15 μM 5-Azacytidin, 10μM Forskolin, 10 nM LY411575 , 5 μM RepSox, and 2 μM CHIR99021 for 2 days, and F12 medium containing 2% FBS for 2 days, and the results of bright field and immunofluorescence are shown in the experimental group. Scale bars: 80 µm. ( d ) Forskolin-treated cells were treated with DMEM/F12 medium containing 8% HS for 3 days, and the results of bright field and Oil red O staining are shown in the experimental group. Scale bars: 80 µm.

    Article Snippet: For Forskolin-treated cells, we used DMEM/F12 culture medium containing 10% fetal bovine serum, 10 ng/mL bFGF, 0.5μM Forskolin (Targetmol, T2939, purity 99.86%, Shanghai, China), and 1 × PS as conditioned medium, and the plate was placed in a 5% CO 2 incubator at 27 °C for culture.

    Techniques: Expressing, Quantitative RT-PCR, Immunofluorescence, Staining