dmem f12 culture medium  (Beijing Solarbio Science)

Journal: Foods

Article Title: Fin Cells as a Promising Seed Cell Source for Sustainable Fish Meat Cultivation

doi: 10.3390/foods14122075

Figure Lengend Snippet: Myogenic and adipogenic induction of TCCF2022 cells. ( a ) The relative expression of Myf5 in TCCF2022 after the addition of Forskolin, LY411575 , RepSox, and CHIR99021, respectively, on the basis of 5-Aza treatment was detected by qRT-PCR. ( b ) The relative expression of Myha in TCCF2022 cells pre-treated with a small-molecule compound after low-serum induced differentiation was detected by qRT-PCR. Statistical analysis was performed on relevant data using one-way ANOVA. Error bars indicate s.d.; ns p ≥ 0.05; * p < 0.05; *** p < 0.0005; **** p < 0.00005. ( c ) Forskolin-treated cells were treated with 15 μM 5-Azacytidin, 10μM Forskolin, 10 nM LY411575 , 5 μM RepSox, and 2 μM CHIR99021 for 2 days, and F12 medium containing 2% FBS for 2 days, and the results of bright field and immunofluorescence are shown in the experimental group. Scale bars: 80 µm. ( d ) Forskolin-treated cells were treated with DMEM/F12 medium containing 8% HS for 3 days, and the results of bright field and Oil red O staining are shown in the experimental group. Scale bars: 80 µm.

Article Snippet: For Forskolin-treated cells, we used DMEM/F12 culture medium containing 10% fetal bovine serum, 10 ng/mL bFGF, 0.5μM Forskolin (Targetmol, T2939, purity 99.86%, Shanghai, China), and 1 × PS as conditioned medium, and the plate was placed in a 5% CO 2 incubator at 27 °C for culture.

Techniques: Expressing, Quantitative RT-PCR, Immunofluorescence, Staining